The correct answer is number: 02 – Anti-H3G34 immunohistochemistry

Explanation:

Diffuse Hemispheric Glioma, H3G34 mutant, CNS WHO Grade 4 is an infiltrative glioma that involves the cerebral hemispheres in children and young adults. The tumour is characterized by a missense mutation of the H3-3A gene at the position G34. This results in the amino acid glycine being replaced by either arginine (H3 G34R mutated) or valine (H3 G34V mutated). The tumor generally has a Glioblastoma-like morphology with highly cellular, infiltrative tumours having brisk mitotic activity. Microvascular proliferation and/or necrosis are usually seen, but are not required for the diagnosis and grading. The histological spectrum can be diverse, with tumors having morphology similar to CNS embryonal tumors or even resembling a low-grade astrocytoma. The prognosis for patients with H3 G34–mutant diffuse hemispheric glioma is generally very poor. The median progression-free survival is 9 months and the median overall survival is 18–22 months (WHO). The current treatment modalities include chemotherapy and radiotherapy.